Dr. Martin Fikáček 團隊使用第三代定序 探索森林落葉層節肢動物多樣性
Fikáček, M., Hu, F.-S., Le, M.-H., Huang, J.-P. 2023. Can immature stages be ignored in studies of forest leaf litter arthropod diversity? A test using Oxford Nanopore DNA barcoding. Insect Conservation and Diversity (early version).
本文刊登於Insect Conservation and Diversity
by Martin Fikáček
In 2018, when I visited Taiwan for the first time and met Fang-Shuo Hu (胡芳碩), we started to discuss the idea of using DNA barcoding to identify all species in our leaf litter samples, to get some ideas about the diversity of leaf litter beetles in Taiwan. We also wanted to include larvae that were always ignored, and I wanted to show that ecological studies are biased a lot by that omission.
We got the first samples in 2019 in Huisun Reserve which Fang-Shuo selected, 4.5 years ago! The first sequences were still done in Prague by Sanger sequencing, but after I moved to Taiwan, we started to use Oxford Nanopore, with the massive support of Jen-Pan Huang and Le Hanh. For me, this paper is really important, as it is my first study addressing biodiversity in other than a taxonomic way, and the first paper in which we use next-gen sequencing.
You may ask what we discovered? In short, the leaf litter beetle diversity in Taiwan is really high, and we continue collecting and barcoding to get more data and a better idea. And, contrary to my guess, we have shown that ecologists may ignore larvae without getting the results biased in many cases: it is always nice to kill one´s idea with real data in hand. For more, read through the paper and wait for the second one that is more taxonomic and on the way!